Pcx^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pcx^{tm1c(EUCOMM)Wtsi}
• Name: pyruvate carboxylase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6382162
• Synonyms: Pcx^f
• Gene: Pcx Location: Chr19:4560500-4671780 bp, + strand Genetic Position: Chr19, 4.07 cM
• Alliance: Pcx^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pcx gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275682
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 4657097 of Chromosome 19 upstream of the critical exon10 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 10 at position 4657983. The critical exon 10 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:275682, J:332296)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcx Mutation: 54 strains or lines available

References

• Original: J:275682 Cappel DA, et al., Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver. Cell Metab. 2019 Jun 4;29(6):1291-1305.e8
• All: 3 reference(s)