Slc6a5^{tm1.1(cre)Ksak}
Targeted Allele Detail

Summary

• Symbol: Slc6a5^{tm1.1(cre)Ksak}
• Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5; targeted mutation 1.1, Kenji Sakimura
• MGI ID: MGI:6382286
• Synonyms: GLYT2^Cre, Slc6a5^{tm1.1(cre)Yyan}
• Gene: Slc6a5 Location: Chr7:49559894-49613604 bp, + strand Genetic Position: Chr7, 31.71 cM, cytoband B3
• Alliance: Slc6a5^{tm1.1(cre)Ksak} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279620
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Slc6a5^{tm1.1(cre)Ksak} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc6a5 (MGI:105090)

• Mutation details: A Cre cDNA, a polyadenylation signal (pA) from the human granulocyte-colony stimulating factor gene, a phosphoglycerate kinase (PGK) promoter-driven neomycin resistance gene (neo)-pA cassette (PGK-neo-pA cassette) flanked by two frt sites were inserted into the translation initiation site (in exon 2) of the mouse Slc6a5 gene by homologous recombination in ES cells. The PGK-neo-pA cassette was removed by crossing mice with FLP66 transgenic mice. (J:279620)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a5 (mouse) Summary of all recombinase alleles driven by Slc6a5. Comparative matrix view of recombinase activities Alliance mouse Slc6a5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc6a5 Mutation: 59 strains or lines available

References

• Original: J:279620 Kakizaki T, et al., A glycine transporter 2-Cre knock-in mouse line for glycinergic neuron-specific gene manipulation. IBRO Rep. 2017 Dec;3:9-16
• All: 3 reference(s)