Fgf18^{tm2.2(cre/ERT2)Dor}
Targeted Allele Detail

Summary

• Symbol: Fgf18^{tm2.2(cre/ERT2)Dor}
• Name: fibroblast growth factor 18; targeted mutation 2.2, David M Ornitz
• MGI ID: MGI:6382298
• Synonyms: Fgf18^CreERT2
• Gene: Fgf18 Location: Chr11:33067430-33097400 bp, - strand Genetic Position: Chr11, 19.21 cM
• Alliance: Fgf18^{tm2.2(cre/ERT2)Dor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279323
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Fgf18^{tm2.2(cre/ERT2)Dor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fgf18 (MGI:1277980)

• Mutation details: A multifunctional targeting vector inserted a splice acceptor (SA)-eGFP:CreERT2 fusion gene into the intron located between Fgf18 exons 1B and 1C along with a PGK-neo selection cassette. Additional Frt and LoxP recombination sites were placed in the targeted allele to allow the generation of two independent alleles by crossing the parental mouse line to either a germline expressed FLP recombinase or a germline expressed Cre recombinase mouse. This generated a tamoxifen inducible eGFP:CreERT2 allele driven by the endogenous Fgf18 promoter (Fgf18CreERT2) that can be used for lineage tracing. The Fgf18CreERT2 allele has exon 1C deleted and is predicted to behave as a functional null allele. (J:279323)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fgf18 (mouse) Summary of all recombinase alleles driven by Fgf18. Comparative matrix view of recombinase activities Alliance mouse Fgf18 gene page

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf18 Mutation: 10 strains or lines available

References

• Original: J:279323 Hagan AS, et al., Generation and validation of novel conditional flox and inducible Cre alleles targeting fibroblast growth factor 18 (Fgf18). Dev Dyn. 2019 Sep;248(9):882-893
• All: 3 reference(s)