Kin^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kin^em1(IMPC)J
• Name: Kin17 DNA and RNA binding protein; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6382547
• Gene: Kin Location: Chr2:10085397-10097512 bp, + strand Genetic Position: Chr2, 6.88 cM, cytoband A1-A3
• Alliance: Kin^em1(IMPC)J page
• IMPC: Kin gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTATCATTAACCCATTTGCA and TCAAAGCTCTCCCCACCTAG, which resulted in a 418 bp deletion beginning at Chromosome 2 position 10,085,644 bp and ending after 10,086,061 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001220233 (exon 2) and 323 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 38 and early truncation 4 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Kin Mutation: 22 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)