Hells^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hells^{tm1c(EUCOMM)Wtsi}
• Name: helicase, lymphoid specific; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6382883
• Gene: Hells Location: Chr19:38919359-38959495 bp, + strand Genetic Position: Chr19, 33.57 cM, cytoband C3-D1
• Alliance: Hells^{tm1c(EUCOMM)Wtsi} page
• IMPC: Hells gene page

Mutation origin

• Mutant Cell Line: EPD0102_4_A04
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 38939858 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 38940603. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neomycin selection cassette, leaving loxP sites flanking the critical exon(s). (J:188991)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hells Mutation: 58 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 6 reference(s)