Tg(Gpx4)#Yana
Transgene Detail

Summary

• Symbol: Tg(Gpx4)#Yana
• Name: transgene insertion, Yasuhito Nakagawa
• MGI ID: MGI:6383118
• Synonyms: Tg(loxP-GPx4)
• Transgene: Tg(Gpx4)#Yana Location: unknown
• Alliance: Tg(Gpx4)#Yana page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gpx4)#Yana expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gpx4 (MGI:104767)	Exons II-VII are floxed

• Mutation details: The transgene construct consists of the mouse DNA including approximately 5 kbp of the promoter region, seven exons and approximately 1 kbp of the 3 region with two loxP sites inserted between exon Ib and exon II, and between exon VII and the marker sequence that was added at the 3 end of the construct. In addition, the NheI site located after the stop codon was replaced with a BamHI site to identify mRNA transcribed from the transgene. Eight lines were generated, with three (Fkappa, Falpha, and Fgamma) rescuing Gpx4 knock-out mice from embryonic lethality, however the pound symbol (#) is used when line is not specified and/or lines are pooled. Cre-mediated recombination can be used to delete exons II-VII. (J:156326)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:156326 Imai H, et al., Depletion of selenoprotein GPx4 in spermatocytes causes male infertility in mice. J Biol Chem. 2009 Nov 20;284(47):32522-32
• All: 6 reference(s)