Slc17a8^{em1(icre/ERT2)Zyliu}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc17a8^{em1(icre/ERT2)Zyliu}
• Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8; endonuclease-mediated mutation 1, Zhiyong Liu
• MGI ID: MGI:6384614
• Synonyms: vGlut3-P2A-iCreER
• Gene: Slc17a8 Location: Chr10:89409882-89457111 bp, - strand Genetic Position: Chr10, 44.99 cM
• Alliance: Slc17a8^{em1(icre/ERT2)Zyliu} page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Slc17a8^{em1(icre/ERT2)Zyliu} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc17a8 (MGI:3039629)

• Mutation details: A plasmid encoding a guide RNA and PAM sequence TGG was designed to introduce a P2A peptide fused to an icre/ERT2 cassette into exon 12, immediately before the stop codon, of the solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8 (Slc17a8) gene. Guide RNA and the cas9 nuclease were introduced into the cytoplasm of C57BL/6N-derived fertilized eggs with well recognized pronuclei. (J:282727)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc17a8 (mouse) Summary of all recombinase alleles driven by Slc17a8. Comparative matrix view of recombinase activities Alliance mouse Slc17a8 gene page

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc17a8 Mutation: 41 strains or lines available

References

• Original: J:282727 Li C, et al., Characterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea. Hear Res. 2018 Jul;364:12-24
• All: 5 reference(s)