A1cf^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: A1cf^{tm1c(EUCOMM)Hmgu}
• Name: APOBEC1 complementation factor; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6384908
• Gene: A1cf Location: Chr19:31846164-31926395 bp, + strand Genetic Position: Chr19, 26.58 cM, cytoband C3
• Alliance: A1cf^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Mutant Cell Line: HEPD0719_3_G08
• Germline Transmission: Earliest citation of germline transmission: J:281365
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31888023 of Chromosome 19 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 31888936. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:281365)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any A1cf Mutation: 39 strains or lines available

References

• Original: J:281365 Nikolaou KC, et al., The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing. Cell Rep. 2019 Oct 8;29(2):283-300.e8
• All: 1 reference(s)