Hprt1^{tm1(tetO-KMT2A/MLLT3)Sguo}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(tetO-KMT2A/MLLT3)Sguo}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Shangqin Guo
• MGI ID: MGI:6385058
• Synonyms: MLL-AF9
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(tetO-KMT2A/MLLT3)Sguo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282726
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^{tm1(tetO-KMT2A/MLLT3)Sguo} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KMT2A (4297)

  Kmt2a

  (MGI:96995)

  Contains N terminal

  human

  MLLT3 (4300)

  Mllt3

  (MGI:1917372)

  Contains C terminal

• Mutation details: The targeting vector was designed with tetO (TRE) promoter, upstream of human KMT2A/MLLT3 (MLL-AF9) cDNA followed by an IRES-deltaNGFR cassette. The KMT2A/MLLT3 translocation T(9:11) contains the AT hook and DNA methyltransferase from KMT2A (1444 amino acids) at the N terminal fused to the 192 C-term amino acids from MLLT3 (breakpoints are variable). MLL-AF9 expression is monitored by the co-expressed deltaNGFR on the cell surface. This construct was inserted into a cassette exchange locus upstream of the hypoxanthine guanine phosphoribosyl transferase (Hprt) gene on the X chromosome using Inducible Cassette Exchange (ICE) recombination. (J:282726)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1277 strains or lines available

Notes

129P2/OlaHsd-derived mutant A17 embryonic stem (ES) cell line (also called A2Lox.cre). These cells carry a doxycycline-inducible cre recombinase ORF, flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene.

References

• Original: J:282726 Chen X, et al., MLL-AF9 initiates transformation from fast-proliferating myeloid progenitors. Nat Commun. 2019 Dec 18;10(1):5767
• All: 1 reference(s)