Hspd1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Hspd1^{tm1c(EUCOMM)Hmgu}
• Name: heat shock protein 1 (chaperonin); targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6385857
• Synonyms: Hspd1^fl
• Gene: Hspd1 Location: Chr1:55116994-55127402 bp, - strand Genetic Position: Chr1, 28.01 cM
• Alliance: Hspd1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Hspd1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283049
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55123466 of Chromosome 1 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 55124394. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:283049)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hspd1 Mutation: 46 strains or lines available

References

• Original: J:283049 Fan F, et al., Deletion of heat shock protein 60 in adult mouse cardiomyocytes perturbs mitochondrial protein homeostasis and causes heart failure. Cell Death Differ. 2020 Feb;27(2):587-600
• All: 2 reference(s)