Tnni3^em1(dre)Bzsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnni3^em1(dre)Bzsh
• Name: troponin I, cardiac 3; endonuclease-mediated mutation 1, Bin Zhou
• MGI ID: MGI:6386758
• Synonyms: Tnni3-Dre
• Gene: Tnni3 Location: Chr7:4521304-4526228 bp, - strand Genetic Position: Chr7, 2.61 cM
• Alliance: Tnni3^em1(dre)Bzsh page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Tnni3^em1(dre)Bzsh expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tnni3 (MGI:98783)

• Mutation details: A cDNA encoding Dre recombinase was iserted into the site encoding the translational start codon of the Krt19 gene, using the CRISPR/Cas9 technology. The woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) were placed after the Dre cDNA sequence to stabilize the Dre-encoding mRNA. (J:278073)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnni3 (mouse) Summary of all recombinase alleles driven by Tnni3. Comparative matrix view of recombinase activities Alliance mouse Tnni3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnni3 Mutation: 14 strains or lines available

References

• Original: J:278073 He L, et al., Enhancing the precision of genetic lineage tracing using dual recombinases. Nat Med. 2017 Dec;23(12):1488-1498
• All: 6 reference(s)