Efcab3^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Efcab3^em1(IMPC)J
• Name: EF-hand calcium binding domain 3; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6388590
• Gene: Efcab3 Location: Chr11:104576533-105008365 bp, + strand Genetic Position: Chr11, Syntenic
• Alliance: Efcab3^em1(IMPC)J page
• IMPC: Efcab3 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATAGGATGCTAAGCTCTCCA and AAAACATCCAGAAGCTTTGG, which resulted in a 269 bp deletion beginning at Chromosome 11 position 104,693,210 bp and ending after 104,693,478 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000353657 (exon 4) and 154 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 63 and early truncation 5 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Efcab3 Mutation: 26 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)