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Thoc3em1(IMPC)J
Endonuclease-mediated Allele Detail
Summary
Symbol: Thoc3em1(IMPC)J
Name: THO complex 3; endonuclease-mediated mutation 1, Jackson
MGI ID: MGI:6388620
Synonyms: Thoc3-
Gene: Thoc3  Location: Chr13:54606650-54616653 bp, - strand  Genetic Position: Chr13, 28.69 cM, cytoband B2
Alliance: Thoc3em1(IMPC)J page
IMPC: Thoc3 gene page
Thoc3em1(IMPC)J/Thoc3em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 as uncompacted morula but not at E7.5. Embryos in vitro hatch from the zona pellucida but form atypical outgrowths with an abnormal inner cell mass and few trophectoderm cells.

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Strain of Origin:  C57BL/6NJ
Project Collection: IMPC
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsThis allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ACAACTTTCCAGATCGCCCA and AATGGGGTGGTAGAGCCCCT, which resulted in a 660 bp deletion beginning at Chromosome 13 position 54,467,578 bp and ending after 54,468,237 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001223112 (exon 2) and 503 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 89 and early truncation 23 amino acids later. (J:188991)
Inheritance:    Not Specified
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Thoc3 Mutation:  19 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory