Hells^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Hells^{tm1d(EUCOMM)Wtsi}
• Name: helicase, lymphoid specific; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6389563
• Gene: Hells Location: Chr19:38919359-38959495 bp, + strand Genetic Position: Chr19, 33.57 cM, cytoband C3-D1
• Alliance: Hells^{tm1d(EUCOMM)Wtsi} page
• IMPC: Hells gene page

Mutation origin

• Mutant Cell Line: EPD0102_4_A04
• Germline Transmission: Earliest citation of germline transmission: J:283594
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 38939858 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 38940603. The critical exon(s) was thus flanked by loxP sites and a "conditional ready" (floxed) tm1c allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neomycin selection cassette, and subsequent breeding to a cre-deleter removed the critical exon(s) yielding this null allele. (J:283594)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hells Mutation: 58 strains or lines available

References

• Original: J:283594 Spruce C, et al., HELLS And PRDM9 form a pioneer complex to open chromatin at meiotic recombination hot spots. Genes Dev. 2020;34:1-15
• All: 2 reference(s)