Gpr63^em2Rstot
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gpr63^em2Rstot
• Name: G protein-coupled receptor 63; endonuclease-mediated mutation 2, Rolf W Stottmann
• MGI ID: MGI:6391253
• Synonyms: Gpr63^Del
• Gene: Gpr63 Location: Chr4:24966407-25009233 bp, + strand Genetic Position: Chr4, 10.53 cM, cytoband A3
• Alliance: Gpr63^em2Rstot page

Mutation origin

• Strain of Origin: FVB

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using CRISPR/Cas9 technology, an 8 bp deletion (ACACAAGT) was created in the FVB strain, resulting in a frameshift and a premature stop codon. The resulting peptide lacks the last 21 wild-type amino-acids and gains five new amino-acids before premature termination, thus disrupting both an ER retention signal and an RGS3 PDZ binding motif. The deletion encompasses SNP rs13477613 (G>T, the last T in the deletion), which is specific to FVB and A/J. (J:282171)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr63 Mutation: 27 strains or lines available

References

• Original: J:282171 Snedeker J, et al., Gpr63 is a modifier of microcephaly in Ttc21b mouse mutants. PLoS Genet. 2019 Nov;15(11):e1008467
• All: 1 reference(s)