Ift74^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ift74^{tm1c(EUCOMM)Hmgu}
• Name: intraflagellar transport 74; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6391391
• Synonyms: Ift74^flox
• Gene: Ift74 Location: Chr4:94502728-94581466 bp, + strand Genetic Position: Chr4, 43.34 cM, cytoband C5
• Alliance: Ift74^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ift74 gene page

Mutation origin

• Mutant Cell Line: HEPD0774_6_H07
• Germline Transmission: Earliest citation of germline transmission: J:277145
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 94509640 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 94510575. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 3. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:277145)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift74 Mutation: 44 strains or lines available

References

• Original: J:277145 Shi L, et al., Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice. Biol Reprod. 2019 Jul 1;101(1):188-199
• All: 2 reference(s)