Tchh^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tchh^em1(IMPC)J
• Name: trichohyalin; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6392072
• Gene: Tchh Location: Chr3:93349637-93356384 bp, + strand Genetic Position: Chr3, 40.51 cM
• Alliance: Tchh^em1(IMPC)J page
• IMPC: Tchh gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTGCACTCACGTTTGTTTTG and GACTATATATGAGTTATGAT, which resulted in a 5805 bp deletion beginning at Chromosome 3 position 93,443,307 bp and ending after 93,449,111 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000427035 (exon 2) and 120 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 50 and early truncation 24 amino acids later. There is a 2 bp insertion (AG) at the deletion site. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tchh Mutation: 60 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)