Adap1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Adap1^{tm1c(EUCOMM)Wtsi}
• Name: ArfGAP with dual PH domains 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6392342
• Synonyms: Adap1^fl
• Gene: Adap1 Location: Chr5:139257631-139311377 bp, - strand Genetic Position: Chr5, 78.27 cM
• Alliance: Adap1^{tm1c(EUCOMM)Wtsi} page

Mutation origin

• Mutant Cell Line: EPD0745_3_A10
• Germline Transmission: Earliest citation of germline transmission: J:284709
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 139293260 of Chromosome 5 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 139294176. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:284709)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adap1 Mutation: 26 strains or lines available

References

• Original: J:284709 Van Duzer A, et al., ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival. Life Sci Alliance. 2019 Dec;2(6)
• All: 1 reference(s)