Yif1b^tm1.2Ics
Targeted Allele Detail

Summary

• Symbol: Yif1b^tm1.2Ics
• Name: Yip1 interacting factor homolog B (S. cerevisiae); targeted mutation 1.2, Mouse Clinical Institute
• MGI ID: MGI:6393258
• Synonyms: Yif1B KO
• Gene: Yif1b Location: Chr7:28937754-28947022 bp, + strand Genetic Position: Chr7, 16.94 cM, cytoband A3
• Alliance: Yif1b^tm1.2Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284998
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A FRT-loxP-neo-FRT-LoxP cassette was inserted upstream exon 2 and a single loxP site was inserted between exon 5 and 6. The frt-flanked neomycin cassette was removed by FLP-mediated recombination, followed by cre-mediated recombination in the germline, removing exons 2-5. Western blot analysis confirmed the complete absence of protein expression in primary cultures of hippocampal neurons derived from E18 homozygous mutant mice. (J:284998)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Yif1b Mutation: 23 strains or lines available

References

• Original: J:284998 Alterio J, et al., Yif1B Is Involved in the Anterograde Traffic Pathway and the Golgi Architecture. Traffic. 2015 Sep;16(9):978-93
• All: 2 reference(s)