Rab32^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rab32^{tm1c(KOMP)Wtsi}
• Name: RAB32, member RAS oncogene family; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6393526
• Synonyms: Rab32^f
• Gene: Rab32 Location: Chr10:10420783-10433951 bp, - strand Genetic Position: Chr10, 3.44 cM
• Alliance: Rab32^{tm1c(KOMP)Wtsi} page
• IMPC: Rab32 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231298
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 10426198 of Chromosome 10 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 2 at position 10427183. The critical exon(s) is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression. (J:231298)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab32 Mutation: 16 strains or lines available

References

• Original: J:231298 Li Y, et al., Analysis of the Rab GTPase Interactome in Dendritic Cells Reveals Anti-microbial Functions of the Rab32 Complex in Bacterial Containment. Immunity. 2016 Feb 16;44(2):422-37
• All: 2 reference(s)