Dnaaf3^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnaaf3^em1(IMPC)J
• Name: dynein, axonemal assembly factor 3; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6393544
• Gene: Dnaaf3 Location: Chr7:4525932-4535452 bp, - strand Genetic Position: Chr7, 2.61 cM
• Alliance: Dnaaf3^em1(IMPC)J page
• IMPC: Dnaaf3 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTAACCCTAGGCCACAGTAG and TCTCTGAAAAATCTTGCCCA, which resulted in a 617 bp deletion beginning at Chromosome 7 position 4,527,750 bp and ending after 4,528,366 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000419696 (exon 5) and 459 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 107 and early truncation 21 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dnaaf3 Mutation: 28 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)