Nlrp4f^em1Lil
Endonuclease-mediated Allele Detail

Summary

• Symbol: Nlrp4f^em1Lil
• Name: NLR family, pyrin domain containing 4F; endonuclease-mediated mutation 1, Lei Li
• MGI ID: MGI:6393576
• Synonyms: Nlrp4f^-
• Gene: Nlrp4f Location: Chr13:65324925-65353530 bp, - strand Genetic Position: Chr13, 34.45 cM, cytoband B3
• Alliance: Nlrp4f^em1Lil page

Mutation origin

• Strain of Origin: CD-1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a 62 bp deletion in exon 2, including the start codon. qRT-PCR, immunoblot analysis and immunofluorescent staining confirmed the absence of endogenous transcript and encoded protein in oocytes from homozygous null females. (J:284116)

Expression

In Mice Carrying this Mutation:	25 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp4f Mutation: 52 strains or lines available

References

• Original: J:284116 Qin D, et al., The subcortical maternal complex protein Nlrp4f is involved in cytoplasmic lattice formation and organelle distribution. Development. 2019 Oct 18;146(20):dev183616
• All: 1 reference(s)