Ndnf^{tm2.1(flpo)Ispgl}
Targeted Allele Detail

Summary

• Symbol: Ndnf^{tm2.1(flpo)Ispgl}
• Name: neuron-derived neurotrophic factor; targeted mutation 2.1, Ivo Spiegel
• MGI ID: MGI:6393604
• Synonyms: Ndnf-IRES-FlpO
• Gene: Ndnf Location: Chr6:65648595-65689307 bp, + strand Genetic Position: Chr6, 30.29 cM
• Alliance: Ndnf^{tm2.1(flpo)Ispgl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268054
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Ndnf^{tm2.1(flpo)Ispgl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ndnf (MGI:1915419)

• Mutation details: The targeting vector was designed to insert an internal ribosome entry site (IRES) fused to a FlpO recombinase sequence into the 3' UTR in exon 4 of the neuron-derived neurotrophic factor (Ndnf) gene. The construct also included a frt-flanked neomycin resistance cassette. After homologous redcombination in ES cells, the resulting chimeric mice were bred with flp recombinase expressing mice to remove the neo cassette. (J:268054)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ndnf (mouse) Summary of all recombinase alleles driven by Ndnf. Alliance mouse Ndnf gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ndnf Mutation: 28 strains or lines available

References

• Original: J:268054 Abs E, et al., Learning-Related Plasticity in Dendrite-Targeting Layer 1 Interneurons. Neuron. 2018 Nov 7;100(3):684-699.e6
• All: 5 reference(s)