Bard1^tm4.2Rjbr
Targeted Allele Detail

Summary

• Symbol: Bard1^tm4.2Rjbr
• Name: BRCA1 associated RING domain 1; targeted mutation 4.2, Richard J Baer
• MGI ID: MGI:6399960
• Synonyms: Bard1^co-rec
• Gene: Bard1 Location: Chr1:71066694-71142300 bp, - strand Genetic Position: Chr1, 35.67 cM
• Alliance: Bard1^tm4.2Rjbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269011
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into an Xmn1 restriction site located approximately 900 bp upstream of the first coding exon and a second loxP site along with a PGK-neomycin resistance cassette flanked by FRT sites was inserted into an AgeI restriction site located approximately 350 bp downstream of the first coding exon. The selection cassette was removed via Flp-mediated recombination leaving exon 1 flanked by loxP sites. The floxed region containg exon 1 was deleted via cre-mediated recombination in the germline. (J:269011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bard1 Mutation: 55 strains or lines available

References

• Original: J:269011 Billing D, et al., The BRCT Domains of the BRCA1 and BARD1 Tumor Suppressors Differentially Regulate Homology-Directed Repair and Stalled Fork Protection. Mol Cell. 2018 Oct 4;72(1):127-139.e8
• All: 1 reference(s)