Rnaseh2a^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rnaseh2a^em1(IMPC)Tcp
• Name: ribonuclease H2, large subunit; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6402027
• Gene: Rnaseh2a Location: Chr8:85683239-85694498 bp, - strand Genetic Position: Chr8, 41.38 cM, cytoband C3
• Alliance: Rnaseh2a^em1(IMPC)Tcp page
• IMPC: Rnaseh2a gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR1534 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of TGGGATCCGGAAGCGATAGT targeting the 5' side and AAGTCAGAGTCGTAACACTT targeting the 3' side of a critical region. This resulted in a 790-bp deletion Chr8:84964971-84965760 (GRCm38). (J:265051)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2a Mutation: 46 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 3 reference(s)