Aco2^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Aco2^em1(IMPC)J
• Name: aconitase 2, mitochondrial; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6402258
• Gene: Aco2 Location: Chr15:81756664-81799338 bp, + strand Genetic Position: Chr15, 38.26 cM
• Alliance: Aco2^em1(IMPC)J page
• IMPC: Aco2 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GCTTTTCCCTCTCACATGTG and AGTGCCATGGAAAGACTGAC, which resulted in a 418 bp deletion beginning at Chromosome 15 position 81,895,108 bp and ending after 81,895,525 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000127355 (exon 3) and 159 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 58 and early truncation 1 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Aco2 Mutation: 67 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)