Rnf43^em2Mage
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rnf43^em2Mage
• Name: ring finger protein 43; endonuclease-mediated mutation 2, Markus Gerhard
• MGI ID: MGI:6403354
• Synonyms: Rnf43^H292R/H295R
• Gene: Rnf43 Location: Chr11:87553913-87626365 bp, + strand Genetic Position: Chr11, 52.17 cM
• Alliance: Rnf43^em2Mage page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Histidine codons 292 and 295 were targeted for mutation to arginine codons with an oligonucleotide donor sequence and an sgRNA using CRISPR/Cas9. This resulted in p.His292Arg and p.His295Arg mutations (plus a silent mutation) in this allele. The mutations in the RING domain lead to transactivation of Wnt signalling. (J:276360)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf43 Mutation: 36 strains or lines available

References

• Original: J:276360 Neumeyer V, et al., Loss of endogenous RNF43 function enhances proliferation and tumour growth of intestinal and gastric cells. Carcinogenesis. 2019 Jun 10;40(4):551-559
• All: 3 reference(s)