Iqcf1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Iqcf1^em1(IMPC)J
• Name: IQ motif containing F1; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6403881
• Gene: Iqcf1 Location: Chr9:106377166-106379449 bp, + strand Genetic Position: Chr9, 57.65 cM, cytoband F1
• Alliance: Iqcf1^em1(IMPC)J page
• IMPC: Iqcf1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GTGTGGATACAGAGCAGCGA and TCTCTCTAACAAATTCCAGG, which resulted in a 1953 bp deletion beginning at Chromosome 9 position 106,500,324 bp and ending after 106,502,276 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000583343 and ENSMUSE00000530264 (exons 2 and 3) and 1410 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 1 and early truncation 3 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Iqcf1 Mutation: 7 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)