Ttktm1.1Kops
Targeted Allele Detail
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Symbol: |
Ttktm1.1Kops |
Name: |
Ttk protein kinase; targeted mutation 1.1, Geert Kops |
MGI ID: |
MGI:6404032 |
Synonyms: |
CiMKiTA |
Gene: |
Ttk Location: Chr9:83716742-83754442 bp, + strand Genetic Position: Chr9, 45.61 cM
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Alliance: |
Ttktm1.1Kops page
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Germline Transmission: |
Earliest citation of germline transmission:
J:286421
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Parent Cell Line: |
IB10/E14IB10 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Conditional ready, Humanized sequence) |
Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: The targeting vector was designed to insert a loxP-flanked cassette containing cDNA encoding exons 17-22 from the gene downstream of the endogenous exon 16. The 3' loxP site was preceded by a stop codon and polyadenylation sequence. Downstream of this cassette, endogenous exon 17 was modified to harbor an ACA to GCA mutation at codon 649, resulting in a threonine to alanine mutation (T649A) similar to that found in human cell lines with a mild chromosomal instability (CIN) phenotype. An FRT-flanked puromycin cassette was also included and then removed by Flp-mediated recombination.
(J:286421)
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Original: |
J:286421 Hoevenaar WHM, et al., Degree and site of chromosomal instability define its oncogenic potential. Nat Commun. 2020 Mar 20;11(1):1501 |
All: |
1 reference(s) |
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