Cdh20^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdh20^em1(IMPC)J
• Name: cadherin 20; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6404179
• Gene: Cdh20 Location: Chr1:104696254-104923206 bp, + strand Genetic Position: Chr1, 49.42 cM
• Alliance: Cdh20^em1(IMPC)J page
• IMPC: Cdh20 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTATTGATATGCACAGGTAT and AAGCTACCTATTGAAGGAGT, which resulted in a 1028 bp deletion beginning at Chromosome 1 position 104940986 bp and ending after 104942013 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000157971 (exon 3) and 733 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 82 and early truncation 6 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cdh20 Mutation: 54 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)