Cyp2d^tm1.2Arte
Targeted Allele Detail

Summary

• Symbol: Cyp2d^tm1.2Arte
• Name: cytochrome P450, 2d region; targeted mutation 1.2, TaconicArtemis
• MGI ID: MGI:6404859
• Synonyms: Del(15Cyp2d22-Cyp2d26)1Arte
• Gene: Cyp2d Location: unknown Genetic Position: Chr15, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241123
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Deletion, Insertion
• Cyp2d^tm1.2Arte involves 37 genes/genome features (Gm25990, Gm27331, Gm27463 ...) View all
• Mutation details: A targeting construct replaced exons 4 and 5 of Cyp2d22 with an FRT site, 9 kb human CYP2D6 promoter, 4.4 kb CYP2D6 genomic DNA, polyadenylation sequence, loxP site, neomycin resistance cassette. The human sequence from RP11-142E17 contains the following polymorphisms: the nucleotide transitions 1023C to T and 2850C to T(leading to the amino acid exchanges T107I and R296C in exon 2 and 6, respectively) and a gene conversion to CYP2D7 in exon 9 (associated with the amino acid exchanges P469A, T470A, H478S, G479A, F481V, A482S, and S486T). A second targeting cassette inserted a hygromycin resistance cassette, loxP site, FRT site, splice acceptor polyA sequence, and ZsGreen reporter gene into intron 4 of Cyp2d26. Cre-mediated recombination removed exons 1 through 3 of Cyp2d22 through exon 5 of Cyp2d26 as well as both selection cassettes. Flp-mediated recombination removed the human sequence. (J:241123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp2d Mutation: 2 strains or lines available

References

• Original: J:241123 Scheer N, et al., Modeling human cytochrome P450 2D6 metabolism and drug-drug interaction by a novel panel of knockout and humanized mouse lines. Mol Pharmacol. 2012 Jan;81(1):63-72
• All: 3 reference(s)