Myo7a^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Myo7a^{tm1b(EUCOMM)Wtsi}
• Name: myosin VIIA; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6405407
• Synonyms: Myo7a^-
• Gene: Myo7a Location: Chr7:97700267-97768731 bp, - strand Genetic Position: Chr7, 53.57 cM
• Alliance: Myo7a^{tm1b(EUCOMM)Wtsi} page
• IMPC: Myo7a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283233
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Cre-mediated excision of the parental Myo7a^{tm1a(EUCOMM)Wtsi} allele resulted in the removal of the promoter-driven neomycin selection cassette and critical exons 10-11 leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in the eye and inner ear. LacZ expresion was not detected. (J:283233)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myo7a Mutation: 118 strains or lines available

References

• Original: J:283233 Calabro KR, et al., A Novel Mouse Model of MYO7A USH1B Reveals Auditory and Visual System Haploinsufficiencies. Front Neurosci. 2019;13:1255
• All: 1 reference(s)