Cbfbtm6Ppl
Targeted Allele Detail
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| Symbol: |
Cbfbtm6Ppl |
| Name: |
core binding factor beta; targeted mutation 6, Pu P Liu |
| MGI ID: |
MGI:6407110 |
| Synonyms: |
CbfbmDE |
| Gene: |
Cbfb Location: Chr8:105897306-105944621 bp, + strand Genetic Position: Chr8, 53.04 cM
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| Alliance: |
Cbfbtm6Ppl page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:252447
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Humanized sequence, Inserted expressed sequence) |
| Mutations: |
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Insertion, Nucleotide substitutions
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Mutation details: Exon 5 was replaced by a construct composed of the first 25 bp of mouse exon 5 fused to the 3' end of the human CBFB/MYH11 cDNA (resulting from a chromosome 16 inversion common in human acute myeloid leukemia subtype M4Eo) containing mutated six charged residues in helices D and E to threonine, serine, or alanine residues: D (NANRRKL to NSNRASL) and E (QRELDEA to QAELTSA). The human cDNA fragment used contained the last 66 bp of human exon 5 and exons 33-42 of MYH11. A bovine growth hormone polyadenylation site and a neomycin cassette were inserted immediately downstream. Western blot confirmed expression of the fusion protein.
(J:252447)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cbfb Mutation: |
36 strains or lines available
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| Original: |
J:252447 Zhao L, et al., The C-terminal multimerization domain is essential for leukemia development by CBFbeta-SMMHC in a mouse knockin model. Leukemia. 2017 Dec;31(12):2841-2844 |
| All: |
1 reference(s) |
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Analysis Tools
