Zc3h10^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zc3h10^{tm1c(EUCOMM)Wtsi}
• Name: zinc finger CCCH type containing 10; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6415552
• Gene: Zc3h10 Location: Chr10:128379429-128383877 bp, - strand Genetic Position: Chr10, 77.01 cM
• Alliance: Zc3h10^{tm1c(EUCOMM)Wtsi} page
• IMPC: Zc3h10 gene page

Mutation origin

• Mutant Cell Line: EPD0577_3_D12
• Germline Transmission: Earliest citation of germline transmission: J:287066
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 128379341 of Chromosome 10 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 128382046. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:173534)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zc3h10 Mutation: 15 strains or lines available

References

• Original: J:287066 Yi D, et al., Zc3h10 Acts as a Transcription Factor and Is Phosphorylated to Activate the Thermogenic Program. Cell Rep. 2019 Nov 26;29(9):2621-2633.e4
• All: 2 reference(s)