Pithd1^tm1Ohi
Targeted Allele Detail

Summary

• Symbol: Pithd1^tm1Ohi
• Name: PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1; targeted mutation 1, Izumi Ohigashi
• MGI ID: MGI:6416147
• Synonyms: PITHD1^-, PITHD1^{tm1(tdTomato)}
• Gene: Pithd1 Location: Chr4:135702913-135714555 bp, - strand Genetic Position: Chr4, 68.34 cM
• Alliance: Pithd1^tm1Ohi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:285040
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NJcl

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing a tdTomato reporter followed by a floxed pgk-neo gene in inverse orientation was inserted in-frame just after the start codon in exon 1 replacing part of exon 1. Quantitative RT-PCR and immunoblot analysis confirmed the absence of expression of wild-type RNA and protein in the thymus and testis of homozygous mice. Reporter expression was detected in the cortical region of the thymus in heterozygous mice. (J:285040)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pithd1 Mutation: 15 strains or lines available

Notes

ES cell = HK3i

References

• Original: J:285040 Kondo H, et al., PITHD1 is a proteasome-interacting protein essential for male fertilization. J Biol Chem. 2020 Feb 7;295(6):1658-1672
• All: 1 reference(s)