Anxa3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Anxa3^{tm1c(EUCOMM)Hmgu}
• Name: annexin A3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6416299
• Synonyms: Anxa3^fl
• Gene: Anxa3 Location: Chr5:96941257-96993825 bp, + strand Genetic Position: Chr5, 47.29 cM
• Alliance: Anxa3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Anxa3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:287184
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 96967819 of Chromosome 5 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 96968616. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele, removing the lacZ and neomycin resistance gene and leaving exon 6 floxed. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:287184)

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Anxa3 Mutation: 30 strains or lines available

References

• Original: J:287184 Huang K, et al., Annexin A3 is necessary for parallel artery-vein alignment in the mouse retina. Dev Dyn. 2020 May;249(5):666-678
• All: 1 reference(s)