Igs7tm86(tetO-CiVS/pHluorin)Hze
Targeted Allele Detail
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Symbol: |
Igs7tm86(tetO-CiVS/pHluorin)Hze |
Name: |
intergenic site 7; targeted mutation 86, Hongkui Zeng |
MGI ID: |
MGI:6436677 |
Synonyms: |
Ai86, Ai86(TITL-ArcLight) |
Gene: |
Igs7 Location: unknown Genetic Position: Chr9, Syntenic
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Alliance: |
Igs7tm86(tetO-CiVS/pHluorin)Hze page
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Allele Type: |
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Targeted (Conditional ready, Inducible, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: The targeting vector contains (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a modified Tet response element (TRE or tetO), a loxP-STOP-loxP cassette, the voltage-sensitive fluorescent protein Butterfly 1.2 coding sequence (referred to as ArcLight - details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site inserted into the Igs7 locus (T1 TIGRE - an intergenic region on chromosome 9 [between AB124611 and Carm1]) using recombinase-mediated cassette exchange (RMCE).
The voltage-sensitive fluorescent protein ArcLight is a fusion of a CiVS [Ciona intestinalis voltage sensor (S1-S4 transmembrane segment, including the voltage-sensing domain in S4)] and the fluorescent protein super ecliptic pHluorin (an eGFP variant differing from eGFP at 9 positions) with an A227D mutation, and exhibits voltage-dependent Forster resonance energy transfer (FRET) upon membrane depolarization. In this strain, the fluorophore is inserted at position A242 within the CiVS.
(J:260362, J:289367)
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Original: |
J:260362 Daigle TL, et al., A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell. 2018 Jul 12;174(2):465-480.e22 |
All: |
2 reference(s) |
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