Gcn1^em1Itohk
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gcn1^em1Itohk
• Name: GCN1 activator of EIF2AK4; endonuclease-mediated mutation 1, Ken Itoh
• MGI ID: MGI:6436699
• Synonyms: Gcn1^-
• Gene: Gcn1 Location: Chr5:115703313-115760713 bp, + strand Genetic Position: Chr5, 56.1 cM
• Alliance: Gcn1^em1Itohk page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to induce double-strand breaks within introns 1 and 2 and excise exon 2, resulting in a frameshift and premature stop codon in exon 3. Homozygous mutant mice only express a short form of the protein, consisting of the N-terminus 12 amino acids and lacking the eEF3-like domain and RWD binding domain. (J:287528)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gcn1 Mutation: 138 strains or lines available

References

• Original: J:287528 Yamazaki H, et al., Ribosome binding protein GCN1 regulates the cell cycle and cell proliferation and is essential for the embryonic development of mice. PLoS Genet. 2020 Apr;16(4):e1008693
• All: 1 reference(s)