Tesmin^em1Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tesmin^em1Osb
• Name: testis expressed metallothionein like; endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6437394
• Synonyms: Mtl5^em1Osb
• Gene: Tesmin Location: Chr19:3438857-3457823 bp, + strand Genetic Position: Chr19, 3.15 cM, cytoband B
• Alliance: Tesmin^em1Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology generated a 316 bp deletion that includes the entire exon 3 resulting in a frameshift. Western blot analysis confirmed the absence of TESMIN-L protein and RT-PCR confirmed the absence of Tesmin-S transcript. (J:288964)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Tesmin Mutation: 23 strains or lines available

References

• Original: J:288964 Oji A, et al., Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Mice. Biol Reprod. 2020 Apr 15;102(4):975-983
• All: 2 reference(s)