Llcfc1^em2Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Llcfc1^em2Osb
• Name: LLLL and CFNLAS motif containing 1; endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6437831
• Synonyms: Sof1 del
• Gene: Llcfc1 Location: Chr6:41661365-41662651 bp, + strand Genetic Position: Chr6, 19.86 cM, cytoband B2
• Alliance: Llcfc1^em2Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:289149
• Parent Cell Line: EGR-G01 (ES Cell)
• Strain of Origin: (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• CRISPR/Cas9 technology targeting exons 1 and 2 generated a 1,024 bp deletion. (J:289149)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Llcfc1 Mutation: 16 strains or lines available

References

• Original: J:289149 Noda T, et al., Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502
• All: 3 reference(s)