Igs7^{tm166(tetO-EGFP*,CAG-tTA2)Hze}
Targeted Allele Detail

Summary

• Symbol: Igs7^{tm166(tetO-EGFP*,CAG-tTA2)Hze}
• Name: intergenic site 7; targeted mutation 166, Hongkui Zeng
• MGI ID: MGI:6441963
• Synonyms: Ai166, TIGRE-MORF
• Gene: Igs7 Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Igs7^{tm166(tetO-EGFP*,CAG-tTA2)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292704
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The reporter contains a cre-dependent, tetracycline-activated tTA2-TRE transcriptional cassette combined with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized G)22(-repeat MORF translation switch, and a farnesylated EGFP fluorescent reporter. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. Specifically, the final vector contains from 5' to 3': FRT2, 2x Ins, TRE promoter, loxP-flanked STOP sequence, a Kozak consensus start site (for strong initiation of translation),two Myc-tags, an optimized G)22(-repeat MORF switch, a farnesylated EGFP fluorescent reporter (EGFP-F), WPRE, pA, 2X Ins, CAG promoter, lox2272-flanked STOP sequence, tTA2, WPRE, pA and an attB-PGK-5';hygro-SD-frt5-SA 3';hygro-SV40pA-attPan FRT site, inserted into the T1 TIGRE locus. (J:292704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs7 Mutation: 70 strains or lines available

References

• Original: J:292704 Veldman MB, et al., Brainwide Genetic Sparse Cell Labeling to Illuminate the Morphology of Neurons and Glia with Cre-Dependent MORF Mice. Neuron. 2020 Aug 5
• All: 2 reference(s)