Slc6a3^{em1(flpo)Hbat}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Slc6a3^{em1(flpo)Hbat}
• Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; endonuclease-mediated mutation 1, Helen Bateup
• MGI ID: MGI:6441980
• Synonyms: DAT-Flp, DAT-P2A-Flpo
• Gene: Slc6a3 Location: Chr13:73684866-73726791 bp, + strand Genetic Position: Chr13, 40.1 cM
• Alliance: Slc6a3^{em1(flpo)Hbat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:311138
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Slc6a3^{em1(flpo)Hbat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc6a3 (MGI:94862)

• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to insert a P2A-Flpo sequence 3' of the stop codon in the final exon (exon 15) of gene sequence. (J:311138)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc6a3 (mouse) Summary of all recombinase alleles driven by Slc6a3. Comparative matrix view of recombinase activities Alliance mouse Slc6a3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc6a3 Mutation: 66 strains or lines available

Notes

(129S6/SvEvTac x C57BL/6NCrl)F1 embryonic stem cells are derived from the Gt(ROSA)26Sor^{tm65.1(CAG-tdTomato)Hze)} mouse line.

References

• Original: J:311138 Kramer DJ, et al., Generation of a DAT-P2A-Flpo mouse line for intersectional genetic targeting of dopamine neuron subpopulations. Cell Rep. 2021 May 11;35(6):109123
• All: 4 reference(s)