Fam151a^em1Jkn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Fam151a^em1Jkn
• Name: family with sequence simliarity 151, member A; endonuclease-mediated mutation 1, Ian J Jackson
• MGI ID: MGI:6442001
• Gene: Fam151a Location: Chr4:106591112-106605489 bp, + strand Genetic Position: Chr4, 49.74 cM
• Alliance: Fam151a^em1Jkn page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two deletions were created in exon 1, 19 bp and 6 bp flanking 16 bp intact sequence, using gRNA 5'-GGGTGCCCTGATGCAGGGTTAGG-3' with CRISPR/Cas9 technology. The double deletion causes a frameshift and subsequent premature stop codon in exon 2. Western blot experiments confirmed the loss of protein expression from this allele. (J:284934)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fam151a Mutation: 35 strains or lines available

References

• Original: J:284934 Findlay AS, et al., Fam151b, the mouse homologue of C.elegans menorin gene, is essential for retinal function. Sci Rep. 2020 Jan 16;10(1):437
• All: 1 reference(s)