Gab3^em1Khoe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gab3^em1Khoe
• Name: growth factor receptor bound protein 2-associated protein 3; endonuclease-mediated mutation 1, Kasper Hoebe
• MGI ID: MGI:6446855
• Synonyms: Gab3^KO
• Gene: Gab3 Location: ChrX:74032151-74128511 bp, - strand Genetic Position: ChrX, 38.12 cM
• Alliance: Gab3^em1Khoe page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 2 was targeted with three shRNA guide sequences (5'-ACACTATCTGGAGTCACTTA-3', 5'-CTACCTAGTAGCCAAGACTG-3', 5'-AGTCATCTGGAAGATGGTGC-3') using CRISPR/Cas9 technology. One founder mouse presented with an insertion of a G nucleotide in exon 2 (c.301_302insG), causing a frameshift and premature termination. (J:291290)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gab3 Mutation: 8 strains or lines available

References

• Original: J:291290 Sliz A, et al., Gab3 is required for IL-2- and IL-15-induced NK cell expansion and limits trophoblast invasion during pregnancy. Sci Immunol. 2019 Aug 2;4(38)
• All: 1 reference(s)