Uts2b^em1(cre)Zak
Endonuclease-mediated Allele Detail

Summary

• Symbol: Uts2b^em1(cre)Zak
• Name: urotensin 2B; endonuclease-mediated mutation 1, Zachary Knight
• MGI ID: MGI:6448061
• Synonyms: Uts2b^2A-Cre, Uts2b^cre
• Gene: Uts2b Location: Chr16:27172072-27188989 bp, - strand Genetic Position: Chr16, 18.93 cM
• Alliance: Uts2b^em1(cre)Zak page

Mutation origin

• Strain of Origin: FVB/N

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Uts2b^em1(cre)Zak expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Uts2b (MGI:2677064)

• Mutation details: CRISPR/Cas9 genome editing is used to insert a T2A-Cre cassette immediately upstream of the endogenous stop codon, a 1kb upstream homology arm, and a 2kb downstream homology arm with a TG to GT mutation introduced into the 3' UTR (114-115bp after stop codon) to avoid cleavage by the sgRNA. The T2A oligopeptide mediates ribosomal skipping and is derived from foot-and-mouth disease virus 2A from the insect Thosea asigna virus. T2A permits cre recombinase to be expressed in frame with Uts2b as a separate polypeptide. (J:291579)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Uts2b (mouse) Summary of all recombinase alleles driven by Uts2b. Alliance mouse Uts2b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Uts2b Mutation: 8 strains or lines available

References

• Original: J:291579 Bai L, et al., Genetic Identification of Vagal Sensory Neurons That Control Feeding. Cell. 2019 Nov 14;179(5):1129-1143.e23
• All: 1 reference(s)