Dnm1l^em1Hise
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dnm1l^em1Hise
• Name: dynamin 1-like; endonuclease-mediated mutation 1, Hiromi Sesaki
• MGI ID: MGI:6448439
• Synonyms: Drp1^exonA-KO
• Gene: Dnm1l Location: Chr16:16130094-16176823 bp, - strand Genetic Position: Chr16, 10.12 cM
• Alliance: Dnm1l^em1Hise page

Mutation origin

• Strain of Origin: (C57BL/6J x SJL/J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s), Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two sgRNAs targeting exon A and downstream were used with CRISPR/Cas9 technology to create an exon A-specific knockout. The resulting allele has a 96 bp deletion that removes most of exon A (which is a 3' extension of exon 2) and some downstream intron sequence. This causes mis-splicing and a frameshift and premature stop codon. Immunoblots confirmed the absence of any peptide containing sequence encoded by the ABCD exons. (J:291773)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnm1l Mutation: 44 strains or lines available

References

• Original: J:291773 Itoh K, et al., Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division. Elife. 2019 Oct 11;8:e44739
• All: 1 reference(s)