Ly6e^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ly6e^{tm1c(EUCOMM)Hmgu}
• Name: lymphocyte antigen 6 family member E; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6448980
• Synonyms: Ly6e^fl
• Gene: Ly6e Location: Chr15:74826900-74831752 bp, + strand Genetic Position: Chr15, 34.29 cM
• Alliance: Ly6e^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291868
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74829855 of Chromosome 15 upstream of the critical exons 3-4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 3 and 4 at position 74832033. The critical exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 3 and 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:291868)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ly6e Mutation: 24 strains or lines available

References

• Original: J:291868 Pfaender S, et al., LY6E impairs coronavirus fusion and confers immune control of viral disease. Nat Microbiol. 2020 Jul 23
• All: 3 reference(s)