Sphkap^tm1.1Nina
Targeted Allele Detail

Summary

• Symbol: Sphkap^tm1.1Nina
• Name: SPHK1 interactor, AKAP domain containing; targeted mutation 1.1, Nobuya Inagaki
• MGI ID: MGI:6449058
• Synonyms: SKIP^-, SKIP-mCherry KI
• Gene: Sphkap Location: Chr1:83233163-83385853 bp, - strand Genetic Position: Chr1, 42.73 cM, cytoband C5
• Alliance: Sphkap^tm1.1Nina page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:272327
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An mCherry-poly(A)-loxp-Neo-loxp construct was inserted into exon 1 of the gene. The loxP-flanked neomycin selection cassette was removed via Cre-mediated recombination. mCherry is expressed under the control of the endogenous promoter. RT-PCR on samples from islets showed that no endogenous transcript is expressed from this allele. Western blot analysis confirmed the absence of protein expression in isolated pancreatic islets from homozygous mutant mice. Immunohistochemical analysis showed that mCherry is detected only in beta-cells, and not in alpha-cells from mutant mice. Incubator two-photon excitation microscopy confirmed that mCherry signal is strongly detected in single beta-cells of mutant mice, but not in wild-type mice. (J:272327)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sphkap Mutation: 87 strains or lines available

References

• Original: J:272327 Wang Y, et al., Sphingosine kinase 1-interacting protein is a novel regulator of glucose-stimulated insulin secretion. Sci Rep. 2017 Apr 10;7(1):779
• All: 2 reference(s)