Spp1^{tm1.1(cre/ERT2)Niba}
Targeted Allele Detail

Summary

• Symbol: Spp1^{tm1.1(cre/ERT2)Niba}
• Name: secreted phosphoprotein 1; targeted mutation 1.1, Nick Barker
• MGI ID: MGI:6450712
• Synonyms: Spp1-eGFP-IRES-creERT2
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^{tm1.1(cre/ERT2)Niba} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292580
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Spp1^{tm1.1(cre/ERT2)Niba} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Spp1 (MGI:98389)

• Mutation details: The following construct was inserted immediately after the ATG start codon: the EGFP fluorescent reporter gene, IRES sequence, a cre-ERT2 fusion gene, and a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination. (J:292580)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spp1 (mouse) Summary of all recombinase alleles driven by Spp1. Comparative matrix view of recombinase activities Alliance mouse Spp1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 43 strains or lines available

References

• Original: J:292580 Tan SH, et al., AQP5 enriches for stem cells and cancer origins in the distal stomach. Nature. 2020 Feb;578(7795):437-443
• All: 1 reference(s)