Tecta^em1(icre)Dbe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tecta^em1(icre)Dbe
• Name: tectorin alpha; endonuclease-mediated mutation 1, Dwight E Bergles
• MGI ID: MGI:6451046
• Synonyms: Tecta-Cre
• Gene: Tecta Location: Chr9:42240915-42311225 bp, - strand Genetic Position: Chr9, 23.63 cM
• Alliance: Tecta^em1(icre)Dbe page

Mutation origin

• Strain of Origin: (C57BL/6 x SJL/J)F2

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Tecta^em1(icre)Dbe expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tecta (MGI:109575)

• Mutation details: CRISPR/Cas9 genome editing is used to insert a codon improved cre recombinase (iCre), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a polyadenylation sequence in place of the start codon of the tectorin alpha (Tecta) gene. (J:286224)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tecta (mouse) Summary of all recombinase alleles driven by Tecta. Alliance mouse Tecta gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community A mouse line with Cre expression limited primarily to the sensory epithelium. Cross to TdTomato line reveals sparse labeling of cells within the temporal bone and spiral ganglion neurons. J:286224

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tecta Mutation: 133 strains or lines available

References

• Original: J:286224 Babola TA, et al., Purinergic signaling in cochlear supporting cells reduces hair cell excitability by increasing the extracellular space. Elife. 2020 Jan 8;9:e52160
• All: 3 reference(s)